| 研究成果： |
主持或参加科研项目：
1.“十三五”国家科技重大专项，2018ZX10731101-002-003，艾滋病疫苗研究，2018/01-2020/12，688.86万元，孔维主持；
2. 吉林省科技厅，20180201001YY，双靶点新型肿瘤治疗性疫苗临床前和临床研究，2018/01-2020/12，110万元，孔维主持；
3. 长春市科技局，17YJ002，双靶点新型肿瘤治疗性疫苗临床前和临床研究，2017/09-2019/09，100万元，孔维主持；
4. 吉林省科技发展计划，20130521001JH，吉林省新型疫苗研究创新团队、2013/01-2015/12，20万元，已结题，主持
5. “十二五”国家科技重大专项，2012ZX10001009-002，具有广谱交叉保护的艾滋病疫苗研究，2012/01-2015/12，1972.73万元，已结题，主持
6. “863”重大项目，2010AA022902，甲型H1N1流感亚单位疫苗的研究，2009/11-2012/12，146.92万元，已结题，主持
7. 国家自然科学基金，20872048，可识别鼻咽癌细胞的PEI/DNA/壳聚糖复合物基因控释载体研究，2009/01-2011/01，40万元，已结题，主持
8. “十一五”国家科技重大专项课题，2008ZX10001-012，创新性艾滋病疫苗的前期研究，2008/10-2010/12，2647万元，已结题，主持
9. 新世纪优秀人才支持计划，NCET-06-0302，抗HIV-1艾滋病疫苗的研究，2007/1-2009/12，50万元，已结题，主持
10. 国家自然科学基金，20674029，HPV16衣壳蛋白分子自组装机理的研究，2007/1-2009/12，29万元，已结题，主持
11. “863”重大项目，2006AA02A220、艾滋病疫苗DM-GPE的人体临床研究，2006/12-2010/12，27万元，已结题，主持
12. “863”重大项目，2005AA2Z3H10总课题号2005AA2Z3H11吉大号，抗艾滋病疫苗和药物临床研究，2005，60万元，已结题，主持
13. 国际合作项目，艾滋病疫苗综合研究，2003/01-2007/01，125万元，已结题，主持
14. “863”重大项目，2003AA219090，抗HIV-1中国流行株艾滋病疫苗的研究，2003/01-2005/01，120万元，已结题，主持
代表性期刊论文：
1.Yan Zhou, Ziyan Wang, Yongqing Xu, Zeqiang Zhang, Rui Hua, Wei Liu, Chunlai Jiang, Yan Chen, Wenying Yang*, Wei Kong*, Optimized DNA Vaccine Enhanced by Adjuvant IL28B Induces Protective Immune Responses Against Herpes Simplex Virus Type 2 in Mice. Viral Immunology, 2017, 30(8):601-614
2.Weiran Wang, Meng Qu, Jiawen Wang, Xin Zhang, Haihong Zhang, Jiaxin Wu, Bin Yu, Hui Wu, Wei Kong*, Xianghui Yu*, Autoubiquitination of feline E3 ubiquitin ligase BCA2. Gene, 2018, 638:1-6
3.Dandan Zhao, Bo Sun, Shiyang Sun, Bin Fu, Chuntian Liu, Dawei Liu, Yanfei Chu, Youlei Ma, Lu Bai, Yongge Wu, Yan Zhou, Weiheng Su, Ali Hou, Linjun Cai, Fei Xu, Wei Kong*, Chunlai Jiang*, Characterization of human enterovirus71 virus-like particles used for vaccine antigens. PLoS One, 2017, 12(7):e0181182
4.Lu Fu, Yingnan Li, Yue Hu, Yayuan Zheng, Bin Yu, Haihong Zhang, Jiaxin Wu, Hui Wu*, Xianghui Yu*, Wei Kong*, A norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in mouse model of Alzheimer’s disease. Scientific Reports, 2017, 7: 41041
5.Lu Fu, Hao Jin, Yongjiao Yu, Bing Yu, Haihong Zhang, Jiaxin Wu, Yuhe Yin, Xianghui Yu, Hui Wu*, Wei Kong*. Characterization of NoV P particle-based chimeric protein vaccines developed from two different expression systems. Protein Expression and Purification, 2017, 130: 28-34
6.Zhen Wang，Bin Yu，Baoming Wang，Jingyi Yan，Xiao Feng，Zixuan Wang， Lizheng Wang，Haihong Zhang，Hui Wu，Jiaxin Wu，Wei Kong*，Xianghui Yu*，A novel capsid-modifiedoncolytic recombinant adenovirus type 5 for tumor-targeting gene therapy byintravenous route, Oncotarget, 2016, 7(30) : 47287-47301
7.Yingnan Li, Lu Fu, Yue Hu, Hao Jin, Yayuan Zheng, Yuhe Yin, Hui Wu*, Xianghui Yu*, Wei Kong*, Establishment of a novel method without sequence modification for developing NoV P particle-based chimeric vaccines, Protein Expression and Purification, 2016, 121: 73-80
8.Haihong Zhang, Chenlu Liu, Fangfang Zhang, Fei Geng, Qiu Xia, Zhenzhen Lu, Ping Xu, Yu Xie, Hui Wu, Bing Yu, Jiaxin Wu, Xianghui Yu*, Wei Kong*. MUC1 and survivin combination tumor gene vaccine generates specific immune responses and anti-tumor effects in a murine melanoma model. Vaccine, 2016, 34(24): 2648-2655
9.Zhen Wang, Bin Yu, Baoming Wang, Jingyi Yan, Xiao Feng, Zixuan Wang, Lizheng Wang, Haihong Zhang, Hui Wu, Jiaxin Wu, Wei Kong*, Xianghui Yu*, A novel capsid-modifiedoncolytic recombinant adenovirus type 5 for tumor-targeting gene therapy byintravenous route, Oncotarget, 2016, 7(30) : 47287-47301
10.Lu Fu,?Yingnan Li,?Yue Hu,?Bing Yu,?Haihong Zhang,?Jiaxin Wu,?Hui Wu*,?Xianghui Yu*,?Wei Kong*, Norovirus P particle: an excellent vaccine platform for antibody production against Alzheimer's disease, Immunology Letters, 2015, 168(1): 22-30
11.Yong Zhang, Lili Cui, Yan Chen, Heng Zhang, Jian Zhong, Yanan Sun, Nianqiu Shi, Chunlei Li, Wei Kong*, Zein-Based Nanofibres for Drug Delivery: Classes and Current Applications, Current pharmaceutical design, 2015, 21(22): 3199-3207
12.Weiran Wang, Jiawen Wang, Meng Qu, Xiaojun Li, Jingyao Zhang, Haihong Zhang,Jiaxin Wu, Bin Yu, Hui Wu, Wei Kong*, Xianghui Yu*, Viral Restriction Activity of Feline BST2 Is Independent of Its N-Glycosylation and Induction of NF-kappa B Activation, PloS One, 2015, 10(9): e0138190
13.Jingcai Lu, Tianxu Sun, Dandan Wang, Yunliang Dong, Man Xu, Hongjia Hou, Franklin T. Kong, Chunsu Liang, Tiejun Gu, Pinxu Chen, Shiyang Sun, Xiuping Lv, Chunlai Jiang, Wei Kong*, Yongge Wu*, Protective Immune Responses Elicited by Fusion Protein Containing PsaA and PspA Fragments, Immunological investigations, 2015, 44(5): 482-496
14.Yang Zang, Dongchuan Du, Na Li, Weiheng Su, Xintao Liu, Yan Zhang, Jianhui Nie, Youchun Wang, Wei Kong*, Chunlai Jiang*, Eliciting Neutralizing Antibodies against the Membrane Proximal External Region of HIV-1 Env by Chimeric Live Attenuated Influenza A Virus Vaccines, vaccine, 2015, 33(32): 3859-3864
15.Peihu Fan, Xiaojun Li, Shiyang Sun, Weiheng Su, Dong An, Feng Gao, Wei Kong*, Chunlai Jiang*, Identification of a Common Epitope between Enterovirus 71 and Human MED25 Proteins Which May Explain Virus-Associated Neurological Disease, Viruses-basel, 2015, 7(4): 1558-1577
16.Chu Wang, Di Liu, Xiaohan Guo, Bin Yu, Hui Wu, Haihong Zhang, Jiaxin Wu, Chunlai Jiang*, Wei Kong*, Xianghui Yu*, Antiviral Activity of a Zymolytic Grain Based Extract on, Evidence-Based Complementary and Alternative Medicine, 2015: 642327
17.Mengshu Wang, Yong Zhang, Bingxue Sun, Yanan Sun, Xin Gong, Yongge Wu, Xizhen Zhang, Wei Kong*, Yan Chen*, Permeability of exendin-4-loaded chitosan nanoparticles across MDCK cell monolayers and rat small intestine, biological & pharmaceutical bulletin, 2014, 37(5): 740-747
18.Yang Zhan, Bin Yu, Zhen Wang, Yu Zhang, Haihong Zhang, Hao Wu, Xiao Feng, Ranshen Geng, Wei Kong*, Xianghui Yu*, A fiber-modified adenovirus co-expressing HSV-TK and Coli.NTR enhances antitumor activities in breast cancer cells, International Journal of Clinical and Experimental Pathology, 2014, 7(6): 2580-2860
19.Jingyao Zhang, Jiaxin Wu, Weiran Wang, Hui Wu, Bin Yu, Jiawen Wang, Mingyu Lv, Xiaodan Wang, Haihong Zhang, Wei Kong*, Xianghui Yu*, Role of cullin-elonginB-elonginC E3 complex in bovine immunodeficiency virus and maedi-visna virus Vif-mediated degradation of host A3Z2-Z3 proteins,Retrovirology, 2014, 11(1): 77
20.Bin Yu, Jianing Dong, Chu Wang, Zhen Wang, Lei Gao, Haihong Zhang, Jiaxin Wu, Wei Kong*, Xianghui Yu*, Trimeric knob protein specifically distinguishes neutralizing antibodies to different human adenovirus species: potential application for adenovirus seroepidemiology, The Journal of general virology, 2014, 95(7): 1564-1573
21.Ye Duan, Tiejun Gu, Xizhen Zhang, Chunlai Jiang, Ruosen Yuan, Zhuang Li, Dandan Wang, Xiaoxu Chen, Chunlai Wu, Yan Chen, Yongge Wu*, Wei Kong*, Negative effects of a disulfide bond mismatch in anti-rabies G protein single-chain antibody variable fragment FV57, Molecular Immunology, 2014, 59(2): 136-141
22.Hui Wu, Haihong Zhang, Yue Hu, Qiu Xia, Chenlu Liu, Yingnan Li, Bin Yu, Tiejun Gu, Xizhen Zhang, Xianghui Yu*, Wei Kong*, Sphere Formation Assay is Not an Effective Method for Cancer Stem Cell Derivation and Characterization from the Caco-2 Colorectal Cell Line Current Stem Cell, Research & Therapy, 2014, 9(2): 82-88
23.Mingyu Lv, Jiawen Wang, Jingyao Zhang, Biao Zhang, Xiaodan Wang, Yingzi Zhu, Tao Zuo, Donglai Liu, Xiaojun Li, Jiaxin Wu, Haihong Zhang, Bin Yu, Hui Wu, Xinghong Zhao, Wei Kong*, Xianghui Yu*, Epitope Tags beside the N-Terminal Cytoplasmic Tail of Human BST-2 Alter Its Intracellular Trafficking and HIV-1 Restriction, Plos one, 2014, 9(10): e111422
24.Bo Sun, Xianghui Yu*, Yuhe Yin, Xintao Liu, Yongge Wu, Yan Chen, Xizhen Zhang, Chunlai Jiang, Wei Kong*, Large-scale purification of pharmaceutical-grade plasmid DNA using tangential flow filtration and multi-step chromatography, Journal of Bioscience and Bioengineering, 2013, 116(3), 281-286
25.Xiaojun Li, Peihu Fan, Jun Jin, Weiheng Su, Dong An, Lin Xu, Shiyang Sun, Yan Zhang, Xiangyu Meng, Feng Gao, Wei Kong*, Chunlai Jiang*, Establishment of cell lines with increased susceptibility to EV71/CA16 by stable overexpression of SCARB2. Virology Journal, 2013, 10: 250
26.Xiaodan Wang, Xiaoying Wang, Haihong Zhang, Mingyu Lv, Tao Zuo, Hui Wu, Jiawen Wang, Donglai Liu, Chu Wang, Jingyao Zhang, Xu Li, Jiaxin Wu, Bin Yu, Wei Kong*, Xianghui Yu*, Interactions between HIV-1 Vif and human ElonginB-ElonginC are important for CBF-β binding to Vif, Retrovirology, 2013, 10(1): 94
27.Mengshu Wang, Yong Zhang1, Jiao Feng, Tiejun Gu, Qingguang Dong, Xu Yang, Yanan Sun, Yongge Wu, Yan Chen*, Wei Kong*, Preparation, characterization, and in vitro and in vivo investigation of chitosan-coated poly (d,l-lactide-co-glycolide) nanoparticles for intestinal delivery of exendin-4,International Journal of Nanomedicine, 2013, 8: 1141-1154
28.Lishuang Zhang, Liang Miao, Xin Gong, Huayan Zhang, Lan Yang, Yuhua Shi, Wei Kong*, Chunlai Jiang*,Yaming Shan*, Multiple antigen peptide mimetics containing gp41 membrane-proximal external region elicit broad neutralizing antibodies against human immunodeficiency virus type 1 in guinea pigs, Journal of Peptide Science, 2013, 19(8): 491-498
29.Hui Wu, Haihong Zhang, Peng Wang, Zhuo Mao, Li Feng, Yuqian Wang, Chenlu Liu, Qiu Xia, Bo Li, Heya Zhao, Yan Chen, Jiaxin Wu, Wei Kong*, Xianghui Yu*, Short-Form CDYLb but Not Long-Form CDYLa Functions Cooperatively with Histone Methyltransferase G9a in Hepatocellular Carcinomas, Genes Chromosomes Cancer, 2013, 52(7): 644-655
30.Yuqian Wang, Haihong Zhang, Chenlu Liu, Hui Wu, Peng Wang, Qiu Xia, Lixing Zhang, Bo Li, Jiaxin Wu, Bin Yu, Tiejun Gu, Xianghui Yu*, Wei Kong*, Enhancement of survivin-specific anti-tumor immunity by adenovirus prime protein-boost immunity strategy with DDA/MPL adjuvant in a murine melanoma model, International Immunopharmacology, 2013, 17(1): 9-17
31.Bin Yu, Jianing Dong, Chu Wang, Yang Zhan, Haihong Zhang, Jiaxin Wu, Wei Kong*, Xianghui Yu*, Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera, Virology, 2013, 437(2): 118–123
32.Wei Liu, Enqi Wu, Xianghui Yu, Lihua Feng, Chunlai Jiang, Xiao Zha, Wei Kong*, Detection of human papillomavirus genotypes associated with mucopurulent cervicitis and cervical cancer in Changchun, China, International journal of gynaecology and obstetrics, 2013, 120(2): 124-126
33.Mingyu Lv, Yingzi Zhu, Jiawen Wang, Haihong Zhang, Xiaodan Wang, Tao Zuo, Donglai Liu, Jingyao Zhang, Jiaxin Wu, Wei Kong*, Xianghui Yu*, Purification of eukaryotic tetherin/Vpu proteins and detection of their interaction by ELISA, Protein Expression and Purification, 2013, 91(2): 112-118
34.Mingyu Lv, Jiawen Wang, Yingzi Zhu, Xiaodan Wang, Tao Zuo, Donglai Liu, Jingyao Zhang, Jiaxin Wu, Haihong Zhang, Wei Kong*, Xianghui Yu*, Overexpression of inactive tetherin delGPI mutant inhibits HIV-1 Vpu-mediated antagonism of endogenous tetherin, Febs Letters, 2013, 587(1): 37-43
获得学术奖励:
1.孔维（3/4），手足口病（EV71/CVA16）致病机理与疫苗研发，吉林省科协、吉林省人力资源和社会保障厅，吉林省自然科学学术成果奖，三等奖，2015（姜春来，苏维恒，孔维，孙世洋）
2.孔维（2/10），艾滋病病毒辅助蛋白与宿主限制因子相互作用研究，吉林省科协、吉林省人力资源和社会保障厅，吉林省自然科学学术成果奖，一等奖，2014（于湘晖，孔维，吴嘉昕，张海红，吴慧，于彬，王晓丹，左陶，吕铭宇，王佳雯）
3.孔维（1/10），HIV感染机制和人体对HIV的免疫保护机制研究，吉林省科学技术厅，吉林省科学技术奖，一等奖，2013（孔维，于湘晖，姜春来，吴永革，陈妍，单亚明，张喜珍，张海红，于彬，吴嘉昕）
4.孔维（1/9），腺病毒载体的预存免疫及解决策略的相关研究，吉林省科协、吉林省人力资源和社会保障厅，吉林省自然科学学术成果奖，二等奖，2013（孔维，于湘晖，吴永革，姜春来，于彬，张喜珍，张海红，吴嘉昕，陈妍）
5.孔维（2/5），宫颈癌HPV感染、病毒基因突变以及DNA疫苗的非病毒载体构建，中国抗癌协会，中国抗癌协会科技奖，三等奖，2009（查晓，孔维，于湘晖，张国楠，乌恩齐）
授权发明专利:
1.孔维、张海红、于湘晖、于永慧，以粘蛋白1和生存素为靶点的肿瘤DNA疫苗和病毒载体疫苗，2015.01.07，中国，ZL200910252427.x
2.孔维、殷玉和、孙博，一种适用于大规模生产质粒DNA的纯化方法，2008.12.03，中国，ZL200610016880.7
3.孔维、张一折、姜春来、吴永革、于湘晖，冻干剂型艾滋病重组痘病毒安卡拉株载体疫苗及其制备方法，2007.06.20，中国，ZL200510016800.3 |
