| 研究成果： |
主要开展了微生物及酶工程领域的研究。基于食品工程、疾病诊断、生物能源及天然产物转化的实际需求，从极端环境中发掘微生物以及酶资源，探讨酶活性、稳定性及底物选择性等分子机制；构建细胞表面展示等筛选技术，进行酶的定向进化及理性设计研究。承担了国家自然科学基金面上项目、国家重点基础研究发展计划（973计划）、国家高技术研究发展计划（863计划）、吉林省科技厅重点科技攻关项目等。发表SCI收录论文20余篇，申请发明专利3项，获得吉林省自然科技进步二等奖一项。
发表论文：
1. Li Yuwei, Bu Mingwei, Chen Peng, Li Xiaohong, Chen Changwu, Gao Gui, Feng Yan, Han Weiwei, Zhang Zuoming, Characterization of a Thermophilic Monosaccharide Stimulated β-Glucosidase from Acidothermus cellulolyticus, Chemical Research in Chinese Universites, 2018, 34: 212-220;
2. Yuwei Li, Junling Wang, Limei Wang, Hao Tong, Mingwei Bu, Gui Gao, Weiwei Han and Zuoming Zhang, The PT/S-Box of Modular Cellulase AcCel12B Plays a Key Role in the Hydrolysis of Insoluble Cellulose, Catalysts 2018, 8, 123;
3. Huan Zhang, Rui Fei, Baigong Xue, Shanshan Yu, Zuoming Zhang, Sheng Zhong, Yuanqi Gao and Xiaoli Zhou, Pnserpin: A Novel Serine Protease Inhibitor from Extremophile Pyrobaculum neutrophilum, Int. J. Mol. Sci. 2017, 18, 113;
4. Rong Liang a, Zuoming Zhang, Songyi Lin, Effects of pulsed electric field on intracellular antioxidant activity and antioxidant enzyme regulating capacities of pine nut (Pinus koraiensis) peptide QDHCH in HepG2 cells, Food Chemistry, 2017, 237:793–802.
5. Ruiwen Yang, Xingfang Li, Songyi Lin*, Zuoming Zhang*, Feng Chen，Identification of novel peptides from 3 to 10 kDa pine nut (Pinus koraiensis) meal protein, with an exploration of the relationship between their antioxidant activities and secondary structure, Food Chemistry, 2017, 219:311–320.
6. Junling Wang, Gui Gao, Yuwei Li, Liangzhen Yang, Yanli Liang, Hanyong Jin, Weiwei Han, Yan Feng, Zuoming Zhang, Cloning, Expression, and Characterization of a Thermophilic Endoglucanase, AcCel12B from Acidothermus cellulolyticus 11B, Int. J. Mol. Sci. 2015, 16, 25080-25095.
7. Wei Bing, Honglei Wang, Baisong Zheng, Feng Zhang, Guangshan Zhu, Yan Feng, Zuoming Zhang, Caldicellulosiruptor changbaiensis sp. nov., a cellulolytic and hydrogen-producing bacterium from a hot spring, International Journal of Systematic and Evolutionary Microbiology, 2015, 65:293–297.
8. Yanyan Chen, Dejun Sun, Yulai Zhou, Liping Liu, Weiwei Han, Baisong Zheng, Zhi Wang, Zuoming Zhang, Cloning, Expression and Characterization of a Novel Thermophilic Polygalacturonase from Caldicellulosiruptor bescii DSM 6725, Int. J. Mol. Sci. 2014, 15, 5717-5729.
9. Tao Lu, Zuoming Zhang, Chi Zhang, Glycosyl rotation and distortion by key residues in Endocellulase Cel6A from Theromobifida fusca, Glycobiology, 2014，24: 247–251.
10. K Wang, J. Tang, Z. Zhang, Y. Gao, G. Chen，Laccase on Black Pearl 2000 modified glassy carbon electrode: Characterization of direct electron transfer and biological sensing properties for pyrocatechol, Electrochimica Acta 2012, 70:112– 117C.
11. Liu, G. Yang, L. Wu, G. Tian, Z. Zhang, Y. Feng, Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering, Protein and Cells, 2011, 2:497-506.
12. J. Cai, Y. Xie, B. Song, Y. Wang, Z. Zhang, Y. Feng. Fervidobacterium changbaicum Lip1: identification, cloning, and characterization of the thermophilic lipase as a new member of bacterial lipase family V. Appl Microbiol Biotechnol. 2011, 89(5):1463-73.
13. Q. Li, G. Li, F. Ma, Z. Zhang, B. Zheng and Y. Feng. Highly efficient ring-opening polymerization of caprolactone catalyzed by a recombinant Escherichia coli whole-cell biocatalyst. Process Biochemistry, 2011, 46(2):477-481.
14. X. Zhang, Z. Zhang, Z. Zhu, N. Sathitsuksanoh Y. Yang, Y.-H. Zhang. The noncellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity. Appl. Microbiol. Biotechnol, 2010, 86(2): 525-533.
15. W. Liu, X. Zhang, Z. Zhang, Y.-H. Zhang, Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability, Applied and Environmental Microbiology, 2010, 76:4914-4917.
16. G Yang, A. Bai, L. Gao, Z. Zhang, B. Zheng, Y. Feng. Glu88 in the non-catalytic domain of acylpeptide hydrolase plays dual roles: Charge neutralization for enzymatic activity and formation of salt bridge for thermodynamic stability. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 2009, 1794(1): 94-102.
17. J. Ma, Q. Li, B. Song, D. Liu, B. Zheng, Z. Zhang, Y. Feng, Ring-opening polymerization of _-caprolactone catalyzed by a novel thermophilic esterase from the archaeon Archaeoglobus fulgidus, Journal of Molecular Catalysis B: Enzymatic 56 (2009) 151–157
18. Y. Wang, R. Wang, Q. Li, Z. Zhang, Y. Feng, Kinetic resolution of rac-alkyl alcohols via lipase-catalyzed enantioselective acylation using succinic anhydride as acylating agent, Journal of Molecular Catalysis B: Enzymatic 56 (2009) 142–145
19. Y. Wang, Q. Li, Z. Zhang, J. Ma, Y. Feng, Solvent effects on the enantioselectivity of the thermophilic lipase QLM in the resolution of (R, S)-2-octanol and (R, S)-2-pentanol, Journal of Molecular Catalysis B: Enzymatic 56 (2009) 146–150
20. Z. Zhang, B. Zheng, Y. Wang, Y. Chen, G. Manco, Y. Feng, The conserved N-terminal helix of acylpeptide hydrolase from archeaon Aeropyrum pernix K1 is important for its hyperthermophilic activity. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 2008, 1784(9): 1176-1183. |
