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生物工程系

高仁钧

日期:2020-08-11 点击数: 来源: 太阳成集团tyc234cc

姓名:

高仁钧

职位:

教授

最高学位:

博士

Email:

gaorj@jlu.edu.cn

电话:

13843142825

工作地点:

生命科学楼233

研究方向:

1) 新酶筛选与开发,特别关注嗜热酶的筛选。

2) 基于蛋白质工程的酶的分子改造与固定化。

3) 酶反应器设计、活性调控、工程化制备和酶催化应用。

教育经历:

1992.09-1996.07 太阳成集团tyc234cc分子生物学系分子生物学专业, 获学士学位

1998.09-2003.12 太阳成集团tyc234cc,获博士学位

2005.09-2007.08 中科经理春应用化学研究所和海王生物 博士后

工作经历:

2000.04-2000.07 日本工业技术院生命工学研究所,访问学者

2001.09-2002.08 太阳成集团tyc234cc分子酶学工程教育部重点实验室,助教

2002.09-2004.08 太阳成集团tyc234cc分子酶学工程教育部重点实验室,讲师

2004.09-2009.08 太阳成集团tyc234cc分子酶学工程教育部重点实验室,副教授

2009.09-现在 太阳成集团tyc234cc分子酶学工程教育部重点实验室,教授, 博士生导师

2010.09-2011.09 丹麦奥胡斯大学分子生物学系,访问学者

荣誉称号:

太阳成集团tyc234cc“唐敖庆学者”领军教授

中国生物化学与分子生物学学会工业生物化学分会常务委员

吉林省生物化学与分子生物学会 理事

研究成果:

承担的项目:

国家自然科学基金:嗜热酰基氨肽酶非特异性催化机制的研究及改造

吉林省自然科学基金:高温糖基化生物反应器的构建

国家重点研发计划项目子课题:基于酶和工程菌改造技术的分布式DNA活字存储阵列研究


2019年以来课题组发表文章:

[1] Zihao Huang, Zhenyu Gu, Xiemuxinuer Abuduwupuer, Deyuan Qin, Yuchen Liu, Zheng Guo*, Renjun Gao*, Engineering non-conservative substrate recognition sites of extradiol dioxygenase: Computation guided design to diversify and accelerate degradation of aromatic compounds, International Journal of Biological Macromolecules, 2024, 264, 130739.

[2] Fang Wang, Yi Ouyang, Pengfei Zhou, Yan Zhang, Renjun Gao*, Bekir Engin Eser * and Zheng Guo*, NIR-accelerated cascade reaction for degradation of organophosphorus compounds by Au/PTE/ZIF-8: cooperative effect and mechanism, Catalysis Science & Technology, 2024, 14, 3424.

[3] Xiuhui Tang, Lijuan Zhang, Mingwang Huang, Fang Wang, Guiqiu Xie, Rui Huo, Renjun Gao*, Selective enhanced cytotoxicity of amino acid deprivation for cancer therapy using thermozyme functionalized nanocatalyst, Journal of Nanobiotechnology, 2024, 22, 53.

[4] Lijuan Zhang, Simeng Ding, Xiuhui Tang, Renjun Gao, Rui Huo* and Guiqiu Xie*, The improved antineoplastic activity of thermophilic L‐asparaginase Tli10209 via site‐directed mutagenesis, Biomolecules, 2024, 14, 686.

[5] Yajing Li, Qi Chen, Siyao Liu, Lin Deng, Shichao Li, and Renjun Gao*, Efficient one-pot synthesis of uridine diphosphate galactose employing a tri-enzyme system, Journal of Agricultural and Food Chemistry. 2024, 72, 3644.

[6] Yiwen Xu, Xiaoran Xuan, Renjun Gao, Guiqiu Xie*, Increased expression levels of thermophilic serine protease TTHA0724 through signal peptide screening in Bacillus subtilis and applications of the enzyme, International Journal of Molecular Sciences, 2023, 24, 15950.

[7] Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Søren Vrønning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Renjun Gao*, and Zheng Guo*, Superanionic solvent-free liquid enzymes exhibit enhanced structures and activities, Advanced. Science. 2022, 9, 2202359.

[8] Abidan Ainiwaer, Ao Li, Xingwang Zhao, Yujiao Xu, Siping Han*, and Renjun Gao*, Site-specific covalent immobilization of Methylobacterium extorquens non-blue laccse melac13220 on Fe3O4 nanoparticles by aldehyde Tag, Catalysts. 2022, 12, 1379.

[9] Abidan Ainiwaer, Yue Liang, Xiao Ye and Renjun Gao*, Characterization of a Novel Fe2+ Activated Non-Blue Laccase from Methylobacterium extorquens, International Journal of Molecular Sciences. 2022, 23, 9804.

[10] Yajing Li, Xue Yang and Renjun Gao*, Thermophilic inorganic pyrophosphatase ton1914 from Thermococcus onnurineus NA1 removes the inhibitory effect of pyrophosphate, International Journal of Molecular Sciences. 2022, 23, 12735.

[11] Fang Wang, Yuchen Liu, Chang Du and Renjun Gao*, Current strategies for real-time enzyme activation, Biomolecules, 2022, 12, 599.

[12] Jiabao Lyu, Jian Zhang, Jingxuan Zhu, Siao Chen, Tao Han, Yan Zhang, Renjun Gao, Guiqiu Xie*, Zheng Guo*, Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope, International Journal of Biological Macromolecules, 2022, 210, 21.

[13] Xiaowen Zhang, Zihao Huang, Dan Wang, Yan Zhang, Bekir Engin Eser, Zhenyu Gu, Rongrong Dai, Renjun Gao*, Zheng Guo*, A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants, Journal of Hazardous Materials, 422 (2022), 126860.

[14] Li Zong, Yan Zhang, Zhengkang Shao, Yingwu Wang, Zheng Guo, Renjun Gao*, Bekir Engin Eser*, Optimization and engineering of a self-sufficient cyp102 enzyme from Bacillus amyloliquefaciens towards synthesis of in-chain hydroxy fatty acids, Catalysts, 2021, 11(6), 665.

[15] Li Zong, Renjun Gao, Zheng Guo, hengkang Shao, Yingwu Wang*, Bekir EnginEser*, Characterization and modification of two self-sufficient CYP102 family enzymes from Bacillus amyloliquefaciens DSM 7 with distinct regioselectivity towards fatty acid hydroxylation, Biochemical Engineering Journal, 2021, 166, 107871.

[16] Jiabao Lyu, Renjun Gao, and Zheng Guo*, Galactosyldiacylglycerols: From a Photosynthesis-Associated Apparatus to Structure-Defined In Vitro Assembling, Journal of Agricultural and Food Chemistry. 2021, 69, 32, 8910.

[17] Fang Wang, Rong Li, Hui Jian, Zihao Huang, Yingwu Wang*, Zheng Guo*, and Renjun Gao*, Design and construction of an effective expression system with aldehyde tag for site-specific enzyme immobilization, Catalysts, 2020, 10(4), 410.

[18] Dongni Liu, Lin Deng, Dan Wang, Wei Li, Renjun Gao*, “Bridge regions” regulate catalysis and protein stability of acylpeptide hydrolase, Biochemical Engineering Journal, 2019, 145, 42.

[19] Wei Li, Dongni Liu, Xu Geng, Zhengqiang Li* and Renjun Gao*, Real-time regulation of catalysis by remote-controlled enzyme-conjugated gold nanorod composites for aldol reaction-based applications, Catalysis Science & Technology, 2019, 9, 2221.

[20] Ye Zhou, Bianca Pérez, Weiwei Hao, Jiabao Lv, Renjun Gao*, Zheng Guo*, The additive mutational effects from surface charge engineering: A compromise between enzyme activity, thermostability and ionic liquid tolerance, Biochemical Engineering Journal, 2019, 148,195.

[21] Ye Zhou, Nykola C. Jones, Jannik Nedergaard Pedersen, Bianca Pérez, S. V. Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Peter Kristensen, Renjun Gao*, and Zheng Guo*, Insight into the Structure and Activity of Surface-Engineered Lipase Biofluids, ChemBioChem, 2019, 20, 1266.

[22] Jiabao Lyu, Zidan Li, Juncheng Men, Ruyun Jiang, Guilin Tang, Ye Zhou, Renjun Gao*, Covalent immobilization of Bacillus subtilis lipase A on Fe3O4 nanoparticles by aldehyde tag: An ideal immobilization with minimal chemical modification, Process Biochemistry, 2019, 81, 63.

[23] Dongni Liu, Wei Li, Xiaoyu Jiang, Shuju Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, Wei Kong, Renjun Gao* and Yaming Shan*, Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer’s disease, Theranostics, 2019, 9(8), 2268.